Viral vectors are one method commonly used for gene therapy. Two


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27.50

Viral vectors are one method commonly used for gene therapy. Two types of viral vectors are retroviruses or adenoviruses. Regarding the delivery and expression of the engineered gene, how do these two systems differ? (10 pts) Assuming you are able to eliminate the negative reactions associated with viral therapy, pick a disease state you would treat with a retrovirus and one you would treat with a adenovirus, and describe why you would pick those particular diseases. (10 pts) You would like to genetically engineer a bacteria so it will glow in the presence of an environmental contaminant. The bacteria you select has the ability to grow in the presence of the contamination and secretes a specific enzyme in response to the contamination. You decide to use a fusion protein based system. What is a fusion protein and how would you construct it (10 pts) Once you have your fusion protein you will need to insert into a plasmid which will serve as the reporter mechanism. Describe how you would construct this plasmid (describe the promotors, binding sites not the actual molecular techniques used to construct the plasmid) in order make the bacteria glow in the presence of contamination. (10 pts)

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27.50