Receptor Pharmacology worksheet. Please help me solve this


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Receptor Pharmacology worksheet. Please help me solve this pharmacodynamics / experimental pharmacology problem. ATTACHMENT PREVIEW Download attachment ProblemSet1.xls A membrane prep was assayed for NET (NE transporter) binding using increasing concentrations of 3H-Nisoxetine in triplicate. Background binding for each reaction was identified in duplicate using 10 mM desipramine Sample tube# 1 2 3 4 5 DPM 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 total bckgrd 837 1329 924 189 168 1530 1398 1737 234 249 5061 4668 3660 867 753 7470 7527 7824 771 873 9552 8763 8547 1290 1242 specific mg protein per Rxn dpm/mg fmol/mg 0.364 0.306 0.377 0.325 0.436 Please answer the following questions: What is the average total dpm for each reaction? What is the average "background" dpm for each reaction? What is the average specific dpm for each reaction? What is the specific dpm/mg for each reaction? calculate the fmol/mg of receptor for each reaction, using the following information: Specific Activity of the 3H-Nisoxetine= 87.2 Ci/mmol There are 2.2 x 10^6 dpm/mCi [Hint: make the units cancel] Show your work for converting dpm/mg to fmol/mg – you can get partial credit even if the answer is wrong Graph the resulting fmol/mg (you can use excel or another graphing program). What is your ballpark estimate of the Bmax? Read more

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