Microsoft Word – 380F15PS7.doc Six steps in a newly discovered


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Microsoft Word – 380F15PS7.doc Six steps in a newly discovered biochemical pathway in Neurospora are shown below. Six strains, each carrying a single mutation (a, b, c, d, e, or f) were independently isolated. Enzyme analysis showed them to be blocked in the steps indicated in the diagram (Capital letters are the intermediates). Assume that all arrows represent enzymes composed of a single polypeptide. (Picture Attached) A. Predict the complementation pattern. B. Predict the growth responses on each compound and intermediates accumulated for all six strains. C. Assuming that complementing mutations assort independently in crosses, what percentage of wild-type progeny are expected from the following matings? (1) a x e (single mutants) (2) a f (double mutant) x d (single mutant) (3) a b (double mutant) x a e (double mutant) (4) a f (double mutant) x d e (double mutant) (4) a f (double mutant) x d e (double mutant) ATTACHMENT PREVIEW Download attachment Untitled.png

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