“Lab 4 Selective Media & Agar” “BIO250L” Student


Question Description:

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Please do all of these sections within this assignment I would need this completed asap! ATTACHMENT PREVIEW Download attachment Lab+4-+Selective+Media+and+Agar.docx “Lab 4 Selective Media & Agar” “BIO250L” Student Name: Click here to enter text. Kit Code (located on the lid of your lab kit): Click here to enter text. “Experiment 1- Selection and Differentiation of Body Inhabiting, Gram-Positive Bacteria” “Table 4: Experiment 1 Growth Conditions” “Surface Tested” “Skin” “Nose” “Countertop/Other” “Control” “Growth (Good or Poor)” “LB/Nutrient “MSA Agar” Agar” Click here to Click here to enter text. enter text. Click here to Click here to enter text. enter text. Click here to Click here to enter text. enter text. Click here to Click here to enter text. enter text. (Red or Yellow)” “Color “Other Observations” Click here to enter text. Click here to enter text. Click here to enter text. Click here to enter text. Click here to enter text. Click here to enter text. Click here to enter text. Click here to enter text. “Post-Lab Questions” “1. What substance in MSA confers selectivity? Why?” Click here to enter text. “2. What substance makes MSA differential? Why?” Click here to enter text. “3. What purpose does phenol red serve in MSA?” Click here to enter text. “4. Why are the nutrient agar plates used in this experiment?” Click here to enter text. “Insert photo of your cultures after incubation with your name clearly visible in the background:” “Lab 4 Selective Media & Agar” “BIO250L” “Experiment 2- Selection and Differentiation of Gram-Negative Bacteria from Liquid Samples” “Table 5: MacConkey Agar Results” “Sample” Click here to enter text. Click here to enter text. Click here to enter text. “Growth” Click here to enter text. Click here to enter text. Click here to enter text. “Colony Color” Click here to enter text. Click here to enter text. Click here to enter text. “Analysis” Click here to enter text. Click here to enter text. Click here to enter text. “Post-Lab Questions” “1. What ingredient(s) makes MacConkey agar selective for Gram-negative bacteria?” Click here to enter text. “Lab 4 Selective Media & Agar” “BIO250L” “2. What types of bacteria are inhibited on MacConkey agar?” Click here to enter text. “3. What ingredient(s) makes MacConkey agar differential?” Click here to enter text. “4. Why is an indicator dye used in MacConkey agar?” Click here to enter text. “5. What are some potentially pathogenic bacteria that are lactose fermenters that will grow on MacConkey agar?” Click here to enter text. “6. What are some potentially pathogenic bacteria that do not ferment lactose that will grow on MacConkey agar?” Click here to enter text. “7. How would you verify that the colonies that grew on a MacConkey agar plate were Gram-negative?” Click here to enter text. “8. Look up the formulation for MacConkey agar. Is this a chemically defined or complex media? Why is that important?” Click here to enter text. “Insert photo of your cultures after incubation with your name clearly visible in the background:” “Lab 4 Selective Media & Agar” “BIO250L” Read more

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