BIO 353 Cell Biology 1. Read the assigned paper (Gustavsson et


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I need help with my cell biology homework, questions are attached below. (A,B,C, D and E). ATTACHMENT PREVIEW Download attachment 2015 BIO 353 Cell Biology Homework Assignment 2 posted.docx BIO 353 Cell Biology 1. Read the assigned paper (Gustavsson et al.) on glycolytic oscillations in yeast. You might also wish to read the Mayevsky and Chance review paper but this is optional and will not directly help you with the questions below. 2. Once you read and understand the material in the paper, embark on completing the assignment by answering the following essay questions: A. NADH is an electron transporter that shuttles electrons from substrates to the mitochondrial electron transport chain. What how might allosteric control of glycolytic enzymes be used as a mechanism that could create oscillations in NADH? (5 pts) B. Why might this control mechanism involve both aerobic and anaerobic glycolysis? Cytoplasmic NADH vs. mitochondrial NADH: Which would show greater swings in oxidationreduction state and why? Propose a way in which you could experimentally observe this. (5 pts) C. Would you expect to see cyclic oscillations of ATP levels? Of pyruvate? Of reduced cytochrome c? Explain. Would you expect to see the ATP oscillations in phase or out of phase with the NADH oscillations? (5 pts) D. Draw a diagram that explains the feedback control processes involved in these oscillations. (9 pts) The diagram must be original (not a copy) and hand drawn, not using computer software. For example, the diagram could consist of a “cause and effect” cycle drawn as a circle with each step leading by an arrow to the next. This diagram should include at minimum: a. allosteric controlled enzymes of the anaerobic glycolysis (grouping them together is ok) b. allosteric controlled enzymes of the tricarboxylic acid cycle (grouping them together is ok) c. NADH d. The mitochondrial electron transport chain e. The H+ gradient across the inner mitochondrial membrane f. The ATP synthetase g. ATP h. ADP E. Write a figure legend that summarizes the mechanisms illustrated in your diagram. 250 word maximum. (9 pts)

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