1 Name Lab, Week # BIOTECHNOLOGY Introduction <Include purpose


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Need help on the below attachment iLab experiment. ATTACHMENT PREVIEW Download attachment BIOS242_W5_Lab_Template.docx 1 Name Lab, Week # BIOTECHNOLOGY Introduction <Include purpose of lab experiment> <brief summary of topic investigating and case studies > <state major finding> Procedure < Include information that the reader would need to repeat your experimental procedure. Do not include any observations or results in this section> Observations and Results Result + or Gram Stain Indole Production Mannitol Fermentation Methyl Red Test Citrate Utilization Appearance after Incubation N/A Additional tests performed are listed below: Result + or – Appearance after Incubation Bacterium identified as: You may want to do some additional tests to conclusively identify your bacterium. Some good ones to do might be lactose and sucrose fermentation, like you did last week. Remember, they use Phenol Red Lactose Broth and Phenol Red Sucrose Broth and yellow= positive and red = negative. After you do those two tests, you may want to do Phenol Red Rhamnose and Phenol Red Xylose, which are set up the same way as the sucrose and lactose tests, they just have different sugars. Be sure to record your test results! Discussion Identify the bacterium – Make sure you look in the Virtual Unknown lab report to make sure you got the right organism. After you have made your identification, look at the lab report. You can do 2 this by going to the View menu in Virtual Unknown and selecting Lab Report. You can double check with me that you are researching the right one. Explain what features of this organism make it an ideal candidate host for your insecticide. (Hint: remember you will need to infect insects with the bacteria to deliver the insecticide.) –Find out where the unknown organism was isolated from. Describe in detail the steps you will take to insert the target gene into the bacterium. – Figure 8.1 of your book should help you with this. Conclusion < Within a few sentences, provide a concluding statement about the results of your laboratory>

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